Advances in Biochemical Engineering Biotechnology, Volume by W. Babel, A. Steinbuechel (Editor)

By W. Babel, A. Steinbuechel (Editor)

Residing platforms synthesize seven various periods of polymers. they supply constitution and shape for cells and organisms, functionality as catalysts and effort garage and hold the genetic details. a majority of these polymers own technically fascinating homes. a few of these biopolymers are already used commercially. This unique quantity of Advances in Biochemical Engineering/Biotechnology contains 10 chapters. It provides an summary of the water insoluble biopolyesters, particularly of the microbially synthesized poly-hydroxyalkanoate (PHA) kin. It reviews the cutting-edge of metabolism, legislation and genetic history, the most recent advances made in genetic optimization of micro organism, "construction" of transgenic crops and in vitro synthesis through purified enzymes. moreover, it describes correct applied sciences and evaluates views bearing on expanding the commercial viability and competitiveness of PHA and discusses functions in medication, packaging, foodstuff and different fields.

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Two Arg residues were found to be essential for the activity. Hydrolysis of p-nitrophenylbutyrate was not affected by this Arg modification whereas cutin hydrolysis was severely inhibited, indicating that the modified arginines were involved in the interaction of the enzyme with cutin. This hypothesis was supported by the experimental verification that phenylglyoxal treatment inhibited the binding of the enzyme to cutin. Presumably the Arg residues interact ionically with the polymer and this interaction is important in the hydrolysis of the insoluble polymer.

80. 81. 82. 83. 84. 85. E. Kolattukudy Moire L, Schmutz A, Buchala A, Yan B, Stark RE, Ryser U (1999) Plant Physiol 119:1137 Garbow JR, Ferrantello LM, Stark RF (1989) Plant Physiol 90:783 Stark RE, Garbow JR (1992) Macromolecules 25:149 Neto CP, Rocha J, Gil A, Cordeiro N, Esculcas AP, Rocha S, Delgadillo I, Pedrosa de Jesus JD, Correia FA (1995) J Solid State NMR 4:143 Gil AM, Lopes M, Rocha J, Neto CP (1997) Int J Biol Macromol 20:293 Stark RE, Sohn W, Pacchiano RA Jr, Al-Bashir M, Garbow JR (1994) Plant Physiol 104:527 Cordeiro N, Aurenty P, Belgacem MN, Gandini A, Neto CP (1997) J Colloid Interface Sci 187:498 Tegelaar EW, Hollman P, van der Vegt ST, Leeuw JW, Holloway PJ (1995) Org Geochem 23:239 Heinen W, van den Brand I (1963) Z Naturforsch B 18:67 Kolattukudy PE (1970) Plant Physiol 46:759 Kolattukudy PE (1970) Biochem Biophys Res Commun 41:299 Kolattukudy PE, Walton TJ (1972) Biochemistry 11:1897 Soliday CL, Kolattukudy PE (1977) Plant Physiol 59:1116 Pinot F, Salaun J-P, Bosch H, Lesot A, Mioskowski C, Durst F (1992) Biochem Biophys Res Commun 184:183 Pinot F, Bosch H, Alayrac C, Mioskowski C, Vendais A, Durst F, Salaun JP (1993) Plant Physiol 102:1313 Tijet N, Helvig C, Pinot F, Le Bouquin R, Lesot A, Durst F, Salaun J-P, Benveniste I (1998) Biochem J 332:583 Walton TJ, Kolattukudy PE (1972) Biochem Biophys Res Commun 46:16 Soliday CL, Kolattukudy PE (1978) Arch Biochem Biophys 188:338 Espelie KE, Dean BB, Kolattukudy PE (1979) Plant Physiol 64:1089 Croteau R, Kolattukudy PE (1974) Arch Biochem Biophys 162:458 Croteau R, Kolattukudy PE (1974) Arch Biochem Biophys 162:471 Croteau R, Kolattukudy PE (1975) Arch Biochem Biophys 170:61 Blée E, Schuber F (1990) J Biol Chem 265:12,887 Blée E, Schuber F (1993) Plant J 4:113 Blée E, Stahl U, Schuber F, Stymne S (1993) Biochem Biophys Res Commun 197:778 Croteau R, Kolattukudy PE (1975) Arch Biochem Biophys 170:73 Kiyosue T, Beetham JK, Pinot F, Hammock BD, Yamaguchi-Shinozaki K, Shinozaki K (1994) Plant J 6:259 Stapleton A, Beetham JK, Pinot F, Garbarino JE, Rockhold DR, Friedman M, Hammock BD, Belknap WR (1994) Plant J 6:251 Blée E, Schuber F (1992) J Biol Chem 267:11,881 Blée E, Schuber F (1995) Eur J Biochem 230:229 Croteau R, Kolattukudy PE (1974) Biochemistry 13:3193 Kolattukudy PE (1973) Lipids 8:90 Dean BB, Kolattukudy PE (1977) Plant Physiol 59:48 Agrawal VP, Kolattukudy PE (1977) Plant Physiol 59:667 Agrawal VP, Kolattukudy PE (1978) Arch Biochem Biophys 191:452 Agrawal VP, Kolattukudy PE (1978) Arch Biochem Biophys 191:466 Schmidt A, Grimm R, Schmidt J, Scheel D, Strack D, Rosahl S (1999) J Biol Chem 274:4273 Roberts E, Kutchan T, Kolattukudy PE (1988) Plant Mol Biol 11:15 Espelie KE, Franceschi VR, Kolattukudy PE (1986) Plant Physiol 81:487 Dean BB, Kolattukudy PE (1976) Plant Physiol 58:411 Pozuelo JM, Espelie KE, Kolattukudy PE (1984) Plant Physiol 74:256 Soliday CL, Dean BB, Kolattukudy PE (1978) Plant Physiol 61:170 Robb J, Lee S-W, Mohan R, Kolattukudy PE (1991) Plant Physiol 97:528 Sijmons PC, Kolattukudy PE, Bienfait HF (1985) Plant Physiol 78:115 Mohan R, Kolattukudy PE (1990) Plant Physiol 921:276 Polyesters in Higher Plants 86.

E. Kolattukudy Moire L, Schmutz A, Buchala A, Yan B, Stark RE, Ryser U (1999) Plant Physiol 119:1137 Garbow JR, Ferrantello LM, Stark RF (1989) Plant Physiol 90:783 Stark RE, Garbow JR (1992) Macromolecules 25:149 Neto CP, Rocha J, Gil A, Cordeiro N, Esculcas AP, Rocha S, Delgadillo I, Pedrosa de Jesus JD, Correia FA (1995) J Solid State NMR 4:143 Gil AM, Lopes M, Rocha J, Neto CP (1997) Int J Biol Macromol 20:293 Stark RE, Sohn W, Pacchiano RA Jr, Al-Bashir M, Garbow JR (1994) Plant Physiol 104:527 Cordeiro N, Aurenty P, Belgacem MN, Gandini A, Neto CP (1997) J Colloid Interface Sci 187:498 Tegelaar EW, Hollman P, van der Vegt ST, Leeuw JW, Holloway PJ (1995) Org Geochem 23:239 Heinen W, van den Brand I (1963) Z Naturforsch B 18:67 Kolattukudy PE (1970) Plant Physiol 46:759 Kolattukudy PE (1970) Biochem Biophys Res Commun 41:299 Kolattukudy PE, Walton TJ (1972) Biochemistry 11:1897 Soliday CL, Kolattukudy PE (1977) Plant Physiol 59:1116 Pinot F, Salaun J-P, Bosch H, Lesot A, Mioskowski C, Durst F (1992) Biochem Biophys Res Commun 184:183 Pinot F, Bosch H, Alayrac C, Mioskowski C, Vendais A, Durst F, Salaun JP (1993) Plant Physiol 102:1313 Tijet N, Helvig C, Pinot F, Le Bouquin R, Lesot A, Durst F, Salaun J-P, Benveniste I (1998) Biochem J 332:583 Walton TJ, Kolattukudy PE (1972) Biochem Biophys Res Commun 46:16 Soliday CL, Kolattukudy PE (1978) Arch Biochem Biophys 188:338 Espelie KE, Dean BB, Kolattukudy PE (1979) Plant Physiol 64:1089 Croteau R, Kolattukudy PE (1974) Arch Biochem Biophys 162:458 Croteau R, Kolattukudy PE (1974) Arch Biochem Biophys 162:471 Croteau R, Kolattukudy PE (1975) Arch Biochem Biophys 170:61 Blée E, Schuber F (1990) J Biol Chem 265:12,887 Blée E, Schuber F (1993) Plant J 4:113 Blée E, Stahl U, Schuber F, Stymne S (1993) Biochem Biophys Res Commun 197:778 Croteau R, Kolattukudy PE (1975) Arch Biochem Biophys 170:73 Kiyosue T, Beetham JK, Pinot F, Hammock BD, Yamaguchi-Shinozaki K, Shinozaki K (1994) Plant J 6:259 Stapleton A, Beetham JK, Pinot F, Garbarino JE, Rockhold DR, Friedman M, Hammock BD, Belknap WR (1994) Plant J 6:251 Blée E, Schuber F (1992) J Biol Chem 267:11,881 Blée E, Schuber F (1995) Eur J Biochem 230:229 Croteau R, Kolattukudy PE (1974) Biochemistry 13:3193 Kolattukudy PE (1973) Lipids 8:90 Dean BB, Kolattukudy PE (1977) Plant Physiol 59:48 Agrawal VP, Kolattukudy PE (1977) Plant Physiol 59:667 Agrawal VP, Kolattukudy PE (1978) Arch Biochem Biophys 191:452 Agrawal VP, Kolattukudy PE (1978) Arch Biochem Biophys 191:466 Schmidt A, Grimm R, Schmidt J, Scheel D, Strack D, Rosahl S (1999) J Biol Chem 274:4273 Roberts E, Kutchan T, Kolattukudy PE (1988) Plant Mol Biol 11:15 Espelie KE, Franceschi VR, Kolattukudy PE (1986) Plant Physiol 81:487 Dean BB, Kolattukudy PE (1976) Plant Physiol 58:411 Pozuelo JM, Espelie KE, Kolattukudy PE (1984) Plant Physiol 74:256 Soliday CL, Dean BB, Kolattukudy PE (1978) Plant Physiol 61:170 Robb J, Lee S-W, Mohan R, Kolattukudy PE (1991) Plant Physiol 97:528 Sijmons PC, Kolattukudy PE, Bienfait HF (1985) Plant Physiol 78:115 Mohan R, Kolattukudy PE (1990) Plant Physiol 921:276 Polyesters in Higher Plants 86.

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